FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l06_n02_SAL5D-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l06_n02_SAL5D-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1407
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40.28429282160625446No Hit
NNAAGTAAGGGGCCGAAGCCCCTGCAATTAAAATTGTTGACCACCTACAT20.14214641080312723No Hit
NNAAGCGGTCTGGAAACGTACGGATTGTTCAGTAACTTGACTCATGATTT20.14214641080312723No Hit
NNCGACAATGTTNACATACAGATCCACGTCTTACAAACATTATAAAGGTC20.14214641080312723No Hit
NNGCTTCCTCCTGAGACTGAGCTTTCTCGCCAAATGACGACTTCTACCAC20.14214641080312723No Hit
NNGAGAAATAAAAGTCTGAAACATGATTAAACTCCTAAGCAGAAAACCTA20.14214641080312723No Hit
NNCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTC20.14214641080312723No Hit
NNAGAATCAGAATGAGCCGCAACTTCGGGATGAAAATGCTCACAATGACA20.14214641080312723No Hit
NNCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAG20.14214641080312723No Hit
NNAGCAGGAAAGCGAGGGTATCCTACAAAGTCCAGCGTACCATAAACGCA20.14214641080312723No Hit
NNCGATTAGAGGCGTTTTATGATAATCCCAATGCTTTGCGTGACTATTTT20.14214641080312723No Hit
NNCAACGGCTGCGGACGACCAGGGCGAGCGCCAGAACGTTTTTTACCTTT20.14214641080312723No Hit
NNTTAACGCTAAAGGTGCTTTGACTTATACCGATATTGCTGGCGACCCTG20.14214641080312723No Hit
NNCGCATAATCTCGGAAACCTGCTGTTGCTTGGAAAGATTGGTGTTTTCC20.14214641080312723No Hit
NNAAGGACTGGTTTAGATATGAGTCACATTTTGTTCATGGTAGAGATTCT20.14214641080312723No Hit
NNGAGGTGATTTATGTTTGGTGCTATTGCTGGCGGTATTGCTTCTGCTCT20.14214641080312723No Hit
NNAACCAACCATCAGCATGAGCCTGTCGCATTGCATTCATCAAACGCTGA20.14214641080312723No Hit
NNGCGTTTACCTTGCGTGTACGCGCAGGAAACACTGACGTTCTTACTGAC20.14214641080312723No Hit
NNCTAATATTCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATC20.14214641080312723No Hit
NNTAAAGGTGAGCCGCTTAAAGCTACCAGTTATATGGCTGTTGGTTTCTA20.14214641080312723No Hit
NNTTTTTTACCTATAGACATTACATCACTCCTTCTGCACGTAATTTTTGA20.14214641080312723No Hit
NNTCCCAATGCTTTGCGTGACTATTTTCGTGATATTGGTCGTATGGTTCT20.14214641080312723No Hit
NNTTTCCAAGCAACAGCAGGTTTCCGAGATTATGCGCCAAATGCTTACTC20.14214641080312723No Hit
NNCGGCTCATTCTGATTCTGAACAGCTTCTTGGGAAGTAGCGACAGCTTG20.14214641080312723No Hit
NNCCGATATTGCGGGCGACCCTGTTTTGTATGGCAACTTGCCGCCGCGTG20.14214641080312723No Hit
NNTTAATCAAGATGATGCTCGTTATGGTTTCCGTTGCTGCCATCTCAAAA20.14214641080312723No Hit
NNTGGACTTTGTAGGATACCCTCGCTTTCCTGCTCCTGTTGAGTTTATTG20.14214641080312723No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)