FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l06_n02_SAL180-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l06_n02_SAL180-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14729621
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG22950.018.1254437
GTCTAGG48250.014.1597223
CCGTATC106200.013.93330194-95
CCTAGAC47800.013.6112925
TATACCG50000.013.1965737
ATAGACG27000.013.0605586
TAGAGCG57800.012.656567
CTAGACA67500.012.648126
GACGGTC29550.012.4588879
GTCTATG61700.011.7509823
GTCTTAG43000.011.6732033
CATAGAC66100.011.460025
AGTACCG46150.011.3965427
CACTGAG86800.011.2922419
GTACCGT35600.011.1474528
TATACTG67000.010.9900087
GACAGTG46200.010.86659059
GTGCTAG49500.010.6098183
CTAGGGC44850.010.5690755
TGGACAG66150.010.5529797
Produced by FastQC (version 0.11.5)