FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l06_n02_SAL180-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l06_n02_SAL180-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9859268
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG33750.015.4238433
CCGTATC126400.014.055119594-95
TAGACGG15350.014.0204227
CGTATCA111800.013.98205494-95
GTCTATG38250.012.394163
TATACCG35600.012.3593267
CTAGACA48450.012.1621316
GTATCAT109350.012.00467894-95
TATACTG42250.011.9987527
TAGAGGT39850.011.8760396
GACAGTG31200.011.6497339
CCAGGAC29600.011.6183425
CCTAGAC35250.011.6018635
TAGACCG23000.011.4364657
TGGTCGC328200.011.38595392-93
TCTAGAC33250.011.3213325
AGAACCG34450.011.1067
GTCTTAG26800.011.0992483
CTAAGGT40600.011.085256
GTCCTAA35850.011.0199123
Produced by FastQC (version 0.11.5)