FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l06_n02_SAL15-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l06_n02_SAL15-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16627421
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC146300.015.9963794-95
TAGACGG24300.015.5402897
TATACCG56850.013.9577677
GTCTAGG57750.013.5217383
GTACCGT38950.013.49968058
AGTACCG51550.013.3527927
GTCTATG67900.012.5957283
TAGAGCG63150.011.9597467
CGTATCA133050.011.88896794-95
GCCGTAT241900.011.82229694-95
TAGAGGT66050.011.6616916
CCTAGAC52250.011.6525845
AGGACAG51800.011.3504647
CACTGAG93200.011.2834969
CGCCGTA264500.011.20675294-95
TATACTG72450.011.1503017
GTGTATG83700.010.9399673
ATAGACG29900.010.8630846
GGTCGCC420750.010.77698394-95
GACAGTG54800.010.7290549
Produced by FastQC (version 0.11.5)