FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l06_n02_SAL15-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l06_n02_SAL15-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20765916
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG58150.018.840867
TAGACGG27950.017.4595627
ATCATTA423000.017.07903594-95
TATCATT505600.016.90347794-95
GTCTAGG66800.016.837263
GTATCAT554900.015.59394994-95
TAGAGCG62350.015.3464727
CCTAGAC62150.015.029575
TCATTAA330000.014.88530294-95
GTCTATG70900.014.8803163
CATTAAA268800.014.76271994-95
GTACCGT45200.014.7126298
CGTATCA622350.014.60075794-95
GGACCGT52400.014.2431898
CTAGACA84050.014.0213716
CCGTATC746450.014.00958194-95
CACTGAG93550.013.9614539
TATACTG92150.013.6544887
TAGACCG39450.013.5826967
AGTACCG54550.013.4187377
Produced by FastQC (version 0.11.5)