FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l06_n02_DHA60-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l06_n02_DHA60-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15126791
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG47000.015.4663477
CCGTATC296950.014.98812694-95
CGTATCA244000.014.44805894-95
GTCTAGG40150.014.2384823
TAGACGG20250.014.1699487
CGCCGTA467700.013.93956494-95
GCCGTAT396200.013.92629794-95
GTATCAT231450.013.80853994-95
TATCATT227950.013.206593594-95
CCTAGAC43350.013.1863365
ATCATTA200600.012.98388194-95
GTATAAG60200.012.738893
TATACTG67300.012.2934287
TCGCCGT616700.012.18127494-95
TCTCGGG113400.012.16913194-95
GTCGCCG871200.011.5991394-95
GGTCGCC855600.011.48261694-95
TACACAG45150.011.4395257
TACACCG36450.011.414687
TAGAGCG41250.011.3617217
Produced by FastQC (version 0.11.5)