FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l06_n02_DHA180-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l06_n02_DHA180-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11096905
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC116250.014.92365594-95
CCTAGAC32550.013.42147355
TATACCG39450.013.3293517
GTCTAGG32700.012.5082223
CGTATCA105150.012.48457294-95
TAGACGG15450.012.0670057
TGGTCGC319000.011.78025292-93
CGCCGTA195750.011.76559894-95
CTAGACA51500.011.7127566
GCCGTAT175350.011.69265394-95
CCTAGGC43850.010.9166915
GTCGCCG366850.010.89339694-95
TCGCCGT260950.010.8434994-95
GACTGCG18200.010.768919
GGTCGCC338850.010.68469194-95
TAGAGCG38200.010.6369937
GTACCGT29300.010.6049618
GTCTTAG31350.010.5264393
GTGTATG49950.010.5148633
GTATCAT106850.010.4408894-95
Produced by FastQC (version 0.11.5)