FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l06_n02_DHA15-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l06_n02_DHA15-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9026840
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG13400.016.7863857
CCTAGAC27850.013.8506945
CCGTATC200350.013.62458294-95
CGTATCA171700.013.46669394-95
TATACCG33650.013.2270197
GTCTAGG23550.013.2232653
TATACTG39700.013.019597
CTAGACA40450.012.6170996
ATCATTA146100.012.38170594-95
GTATCAT163900.012.14071894-95
CTCTAGA46850.012.1034014
GGACTGC26250.012.0330658
TATCATT156650.011.9771794-95
GACGGTC16900.011.8938989
GCCGTAT258150.011.73294694-95
GTACCGT22050.011.7205178
GTCTAAG26300.011.4871533
GTGTATG43750.011.473623
TAGACTG25550.011.4262317
CGCCGTA293700.011.34720494-95
Produced by FastQC (version 0.11.5)