FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l06_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l06_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16642421
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACATA7050.025.65973726-27
GAGAGTC8500.019.3033124-25
GTCGATA9950.019.02723124-25
TGCGATT11500.019.02355424-25
TGGTCGA9900.018.69836422-23
TTACGTT7900.018.63919324-25
GTCGACA8750.018.27097324-25
CTTAGTC7150.018.24071324-25
CGCATAG6050.018.08023622-23
CAATCGT7800.017.79940422-23
ATGCTAG10650.017.38157822-23
GCTAGCG7800.017.26002924-25
CGATTAT9600.017.0909526-27
TAACGGG3755.4569682E-1116.8285324-25
CATAGCG6500.016.8285322-23
TTACGGT8750.016.8285324-25
TTTACGT10550.016.74877224-25
ACGATCA9800.016.74266824-25
AACGTGT5550.016.6769222-23
TTGGACG8100.016.6207722-23