FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l06_n01_SAL60-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l06_n01_SAL60-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13671613
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG20300.018.7382225
TATACCG48100.017.5110845
CCTAGAC47200.016.3115393
GTCTAGG48150.016.2714671
GTCTATG55550.015.7344011
ACTGTCC34100.015.670718
CTAGACA64950.015.338934
TAGAGCG53100.014.9241195
GTACCGT33450.014.4854246
GTATAGG62950.013.8847641
AGACACT41250.013.7223856
ACTGAGC73950.013.411188
GACGGTC25550.013.1159187
TTCTGCT175400.012.98617394-95
GTCCTAA46850.012.95305251
GACTGCG15800.012.897737
GACTGTG20400.012.8752517
TAGACAC54250.012.7713515
CACTGAG77500.012.6799027
AGTACCG44300.012.6754425
Produced by FastQC (version 0.11.5)