FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l06_n01_SAL60-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l06_n01_SAL60-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8331848
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGAC29850.016.3760533
CTAGACA39650.015.5238514
TGCCGTC297950.015.09876894-95
GCCGTCT239800.014.99488994-95
TATACCG25150.014.9358815
TAGAGCG29550.014.0903265
ACTGTCC23650.013.5874768
GTCTAGG31000.013.5783151
TATACTG38550.013.5009455
CCGTCTT194800.013.30102494-95
GACTGCG14350.013.2467387
AGTACCG25300.013.0584935
TAGAGGT33950.012.9311074
GTACCGT21750.012.9009156
ATGCCGT325450.012.85645394-95
TAGACGG14950.012.7144865
TACACTG26450.012.66183955
TAAGGCG13600.012.6454835
TTAGGAC30100.012.4807713
GGACTGC26150.012.4608866
Produced by FastQC (version 0.11.5)