FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l06_n01_SAL5D-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l06_n01_SAL5D-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18563581
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC554150.013.26167394-95
GCCGTCT456500.012.900694-95
CCGTCTT373300.012.15625494-95
GTCTTCT318250.011.79835294-95
ATGCCGT587550.011.77589694-95
CGTCTTC369100.011.6997894-95
TATGCCG643400.011.37264892-93
TATACTG92800.09.8530425
TTCTGCT238800.09.82851394-95
CGTATGC752300.09.76547190-91
GTATGCC723350.09.64632792-93
GTATAAG66450.09.197061
GTATTGG101150.08.86154651
CTTCTGC256400.08.8537394-95
ACTGCGC45300.08.7933858
TCGTATG795450.08.78049190-91
CTCGTAT852450.08.57299488-89
TCTTCTG298350.08.5115894-95
GTGTAGG69450.08.4738611
TACACTG71150.08.4614285
Produced by FastQC (version 0.11.5)