FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l06_n01_SAL180-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l06_n01_SAL180-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14729621
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG23300.018.4546975
TAGAGCG61500.015.9706965
CCTAGAC49300.015.5174483
GTCTAGG52050.015.2189981
TAGAGGT62150.013.91023354
TATACTG63750.013.1350215
GTCTATG59000.013.1193651
AGACACT42800.013.1132346
GTCCTGG39050.012.9827211
CTAGACA67750.012.9608844
GCCGTCT222600.012.62703194-95
ATAGACG26800.012.4979594
GTACCGT37050.012.4607396
TGCCGTC268650.012.24006294-95
GTGGTAG84000.012.2325031
TATACCG48350.012.1698755
ACTGTCC39750.012.0699798
CATAGAC64150.011.9253353
ACTGAGC78100.011.8227068
GACTGTG23950.011.7172037
Produced by FastQC (version 0.11.5)