FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l06_n01_SAL180-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l06_n01_SAL180-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9859268
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG13900.018.2323235
TATACCG32500.015.8741445
GTCTAGG38100.015.3238031
TAGAGCG39350.014.260865
CCTAGAC31750.014.2550613
GCCGTCT266350.013.77153494-95
CTAGACA47000.013.6729964
TGCCGTC321750.013.671926594-95
ACTGTCC27500.012.671858
TAGAGGT38500.012.5775564
TATACTG43300.012.4373495
ACTGAGC56650.012.2228798
GCTATAG44550.012.1908891
CCGTCTT220250.012.15404594-95
GCCCTAG29600.011.9262721
TCTTCTG166800.011.8839394-95
GTCCTAA35200.011.8289711
CACTGAG55700.011.7811057
GTCTTCT185950.011.77961794-95
ATGCCGT347950.011.71248194-95
Produced by FastQC (version 0.11.5)