FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l06_n01_SAL15-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l06_n01_SAL15-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16627421
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG25550.016.6639295
TATACCG53100.016.2068845
GTCTAGG56400.015.7419751
ACTGTCC44200.014.4491148
TATACTG69500.013.8814625
CCTAGAC58050.013.8121023
GTCTATG65050.013.6486911
GTACCGT42350.013.5826416
GCCGTCT317350.012.69534994-95
ACTGAGC91550.012.6656068
CCGTCTT273050.012.64008494-95
CTAGACA78550.012.6282624
CGTCTTC262300.012.527842594-95
TGCCGTC389100.012.51366194-95
GTCTTCT237700.012.29997494-95
TAGAGCG68150.012.0961175
ATGCCGT412650.011.92024494-95
TAGAGGT70000.011.8412814
AGTACCG54000.011.826765
CCTCTAG70150.011.7523831
Produced by FastQC (version 0.11.5)