FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l06_n01_SAL15-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l06_n01_SAL15-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20765916
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGCT580600.017.83601494-95
GTACCGT43000.017.2653336
TCTGCTT485750.017.25538394-95
GTCTAGG63900.017.14611
TATACCG58550.016.7008255
TGCTTGA265800.016.60593494-95
GCTTGAA199850.016.4907794-95
CCTAGAC65500.016.174493
CTGCTTG349600.015.98576394-95
TAGAGCG62350.015.9733975
CTAGACA87050.015.9657394
TAGACGG29900.015.8975125
ACTGAGC90700.015.7723388
CACTGAG92600.015.2040917
AGTACCG54300.014.839915
CTTCTGC708600.014.78664894-95
GTCTATG71850.014.7448341
TATACTG91500.014.5952655
TACCGTG50500.014.1637027
ACTGTCC51350.014.1057398
Produced by FastQC (version 0.11.5)