FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l06_n01_DHA60-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l06_n01_DHA60-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15126791
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG47700.015.3762345
TTCTGCT266050.014.48182394-95
GCCGTCT683150.014.25214894-95
CCGTCTT558950.013.93196694-95
TGCCGTC808900.013.66808894-95
TCTGCTT233300.013.61350294-95
ACTGTCC35450.013.5379548
CGTCTTC495050.013.30671594-95
GTCTTCT420400.013.02028794-95
CTTCTGC315150.012.972609594-95
GTCTAGG41000.012.9205981
CTAGACA61500.012.8094884
CCTAGAC44400.012.64539053
TAGACGG23750.012.5815365
ACTGAGC65000.012.4681818
CATAGAC57900.012.4340353
CTGCTTG175550.012.17301394-95
GTATAGA63250.012.0262271
TCTTCTG370700.011.96296394-95
ATGCCGT896850.011.93143794-95
Produced by FastQC (version 0.11.5)