FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l06_n01_DHA180-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l06_n01_DHA180-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11096905
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG33750.017.1764851
GCCGTCT265400.014.2648294-95
TGCCGTC316600.014.00337694-95
CCTAGAC31600.013.6155333
TAGACGG16950.012.8239185
CCGTCTT218050.012.66877794-95
TATACCG39400.012.5279355
GTCTATG44100.011.9129021
CGTCTTC207350.011.81555794-95
ATGCCGT341900.011.73533694-95
CTAGACA49600.011.686474
TATGCCG384950.011.511065592-93
GTCTTCT185850.011.37929394-95
TAGAGCG39400.011.1487135
TAAGGTG50850.010.9537645
AGTACCG34200.010.7252675
CACTGAG58300.010.6413657
GTGTATG53300.010.621381
TAGACAC40400.010.0881225
TCTTCTG168050.010.05150994-95
Produced by FastQC (version 0.11.5)