FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l06_n01_DHA15-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l06_n01_DHA15-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9026840
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGAC25950.014.9986093
GTACCGT21000.014.4372766
GCTTGAA77800.013.87217394-95
AGTACCG25450.013.8688795
TAGACGG13750.013.8223275
GTGTATG44800.013.4356511
GTCTATG32900.013.3432271
TGCTTGA96800.013.27635194-95
TATACTG39200.013.275365
GTCTAGG27000.013.2418411
TAGAGCG30550.013.1829725
CTAGACA38050.013.0820254
CTGCTTG119950.013.05303894-95
CCAGGGT23300.013.0139393
TATACCG29300.012.818735
TAGGGAG32900.012.7915045
TCTGCTT159150.012.76660694-95
TTCTGCT174250.012.71205194-95
ACTGAGC42250.012.5312998
GCCGTCT392750.012.48960994-95
Produced by FastQC (version 0.11.5)