FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l05_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l05_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16312439
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTCG3206.2645995E-9195.283451
GCGAATG2655.744005E-7188.651171
GAGACCA4701.8189894E-12186.142521
TAACGTG3001.0655622E-6166.641882
ACACGTT3201.4694124E-6156.226762
AGTCTAA5757.2759576E-12152.151262
GATCACG3301.7126731E-6151.49261
AGTTCTG7350.0136.034181
ACGTTCA3803.4560198E-6131.559371
GACGTTA1900.009482814131.559372
CTAGCGT2851.811651E-4131.559371
GGACCAT4857.507515E-8128.846821
GCGTGAT2952.0782874E-4127.0997242
AGGTCAG4958.479583E-8126.2438352
AGATACG4054.7447156E-6123.438421
GGAACGT5101.01326805E-7122.530791
CGTGAAT4105.0432427E-6121.9330751
TAGTCGT3102.531907E-4120.9497451
GTTCTGA8301.8189894E-12120.463992
CGAATGC3152.6983878E-4119.02991
Produced by FastQC (version 0.11.5)