FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l05_n02_DHA60-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l05_n02_DHA60-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23077753
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGTA27200.063.3121831
GCGTATA42850.034.4475671
ACGTTAT20554.2848967E-429.9285951
GCGTCTA37452.3375833E-829.5609841
GCGTTCA54101.8189894E-1229.5579451
GCCCCAA89150.028.9751782
CGCCTAA36906.7703695E-726.6680832
GAGTCCC53159.695214E-1025.4576051
GAGTCTA58301.03682396E-1025.3186681
CGGTTAG34551.2250408E-524.9217282
CGTATAG54601.3242243E-924.7815362
GGCTCCA61001.8189894E-1024.1980061
ACGTTCA30651.55775E-424.079581
GGACCTA41101.7324128E-623.9428751
CGTGTAA36101.7209864E-523.851682
GCGTACT52252.253546E-823.5419181
GCGGTTA49603.5498306E-722.3197351
GCGTACC28350.0027981821.6942731
GCGCATA34453.4365128E-421.4234891
ACGTATA69008.4764906E-1021.3924391
Produced by FastQC (version 0.11.5)