FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l05_n02_DHA180-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l05_n02_DHA180-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11030287
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGTA12553.4307595E-758.7648621
GCGTCTA14505.5337216E-542.3850021
CGGCCTA20602.1723099E-741.7677462
CGTCCTA17853.8905273E-641.316472
CCGTTAA15458.044733E-539.7788051
CGTGGTA15809.1796544E-538.897632
ACGTTCA16201.0635707E-437.937191
GCGTCAT16951.38813E-436.2585561
AGTCGTG18152.0751492E-433.8612982
GCGTGTT15550.00397768431.6183951
CGGCGTA20103.7764278E-430.5762442
CGTGTTA16150.004787191330.4437162
GCGTATT33002.559882E-729.797941
CCGTGTA16800.005805745329.2658351
CGCTGTA17400.00689125328.2566682
GACCTCT39150.028.2566681
GCGTTAT21801.1959128E-728.1918581
CGCCATA22357.027194E-427.49812
GCGTTCA27357.210611E-526.9652291
CGGTCTA23308.961626E-426.3769341
Produced by FastQC (version 0.11.5)