FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l05_n02_DHA15-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l05_n02_DHA15-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10214226
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCTA15052.4556357E-1065.4494861
GCGTATC14358.552397E-751.4816061
CGTCTAG16901.4551915E-1150.9992832
GCGTTAT20904.5147317E-947.1298941
GCGTGTT13500.001972316536.482031
CGTGTAC19102.771439E-432.2321552
GACTTCA53900.031.9809991
CGCTATA19453.0829635E-431.6521452
CAGTCTA19503.1297366E-431.5709861
CTGGCGT16750.005673659529.4034251
ACGCATA16800.005756906729.3159141
CGTCCTA16850.00584111729.2289242
CGTCTTA26055.114269E-528.3593482
GCGTTCA26105.1815452E-528.3050211
CGGTATT27006.527626E-527.361521
GCGTATA18500.00921562826.622021
GGACCCT23709.798105E-425.9761281
CGTACTA29401.1648327E-425.1279282
GCGTACT24900.001306880424.7242661
GAGCGTA30301.4294365E-424.3815541
Produced by FastQC (version 0.11.5)