FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l05_n02_BG760-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l05_n02_BG760-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8105255
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG17408.749339E-1056.708332
CGTCCTA11801.6037151E-552.2629782
GCGTATA14509.07281E-751.03751
ACGCTAA7300.00798213850.6879271
GCGCATA12902.7168328E-547.8064421
CGTCTAG13301.2831151E-846.3686562
ACGTGTA15056.74954E-540.976951
AGACCCA22153.731475E-738.9789771
GAGCCTA23055.098609E-737.457021
GGCCCCA27404.8448783E-836.011861
GGACCCT17151.456542E-435.9593661
CGCTATA10500.002010960135.2401771
GCGCTCA18652.383967E-433.0671921
GACCCTT27802.2061176E-631.0569921
CGTGCTA28102.3987523E-630.7254222
CGATTAT16100.004634433430.6436311
GCGGGTA21255.123472E-429.0213221
GCCCTTA26105.117206E-528.3541642
GCCCCAG17450.00686909228.2729232
ACGTCTA17650.007262083727.9525491
Produced by FastQC (version 0.11.5)