FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l05_n02_BG760-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l05_n02_BG760-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17990477
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGTA17305.3520125E-849.9203151
GCGTCTA22451.5097612E-1049.459711
GGACCTA31459.640644E-1139.228771
GCGTATA35350.038.3909341
CGTCCTA26603.734931E-837.105112
CGTCTAG27304.6946298E-836.1536982
GCGTACA27655.2514224E-835.6960561
CGCTATG22752.0029122E-532.5383262
CGTGTAA34801.1106749E-831.9071982
CGATGTA49901.4551915E-1129.6692181
GCGTCAA30354.372092E-628.4554021
GAGTCTA43703.255991E-928.232151
CGGTATG30704.7800822E-628.1309911
GCGTATT57703.6379788E-1227.796681
CGCCTAA27006.4460226E-527.4165532
ACGCTAT27457.2137474E-526.9671021
ACGGGTA41706.44377E-826.6275881
GAGCCCA65800.026.249891
AGTGTAC37808.1356666E-726.1110042
GCGTGCT44201.1324846E-725.1215041
Produced by FastQC (version 0.11.5)