FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l05_n02_BG75D-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l05_n02_BG75D-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14481499
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAT20103.1614036E-949.0716931
CGCTATA23301.1674274E-842.3322332
GCGTACG15758.852754E-539.1405141
GCGTATA27001.776425E-836.5311471
GCGTGTT14350.002644658634.3672831
CGTTCAA35651.412991E-831.1257692
CGTGTTA17300.006602552328.5069682
GGCTCCA31105.3113235E-627.7507511
CGTACGG17800.00758786227.7062052
ACGGTCA18150.00834409427.1719281
GCGTTCA36556.09758E-726.9860741
GCGCTAT18502.7522723E-526.6578661
ACGCTTA18550.00927996326.586011
GCGCCCA37958.4655403E-725.990541
CGCATAA33509.462183E-625.7626362
AGTCTTA43559.8680175E-825.4795322
GGACATA46551.8792161E-723.8374581
GCCTTAA31250.023.6721862
GACCCAG37402.2204595E-523.0761592
GCGTTCT43752.919005E-622.5449371
Produced by FastQC (version 0.11.5)