FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l05_n02_BG75D-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l05_n02_BG75D-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8792404
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGTA10555.775282E-446.826281
CACCGTA10756.335324E-445.9550932
GAGTCTA21605.8753358E-945.7423361
GCGTATA16009.6073505E-538.59511
GTGCCCA28105.981201E-835.161371
GCGTACT18750.032.9344831
GCGGTTT23902.7813769E-531.0052661
CGTCCTG17150.00627129928.805672
GCGCTTA17600.007116836528.0691621
GAGGCTT26956.310131E-527.496321
GCGCTCC22507.103294E-427.4454021
GGACCAT18101.4106263E-827.293771
GACCTTA13704.3769338E-527.0447391
GACTCCG18650.00944178526.488862
GCCCTAA18750.00969113226.3475862
GAGTCAT33008.2955285E-626.1978841
AGACACA23900.001010532825.8377231
GAGCTTA28709.681401E-525.8197151
GAGAGTA29051.0512737E-425.5086351
CGGTTTA24500.001167774925.2049622
Produced by FastQC (version 0.11.5)