FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l05_n02_BG7180-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l05_n02_BG7180-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9779360
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGTA5953.236706E-583.853141
ACGTTAT6350.004399239658.928291
GCGTATA20455.4569682E-1154.8940771
GCGTCTA10956.6086795E-445.5640341
CGTATAG25104.0745363E-1044.7244572
ACGGGCA13954.0385305E-544.706651
GTCCCTA11608.7791705E-443.0108761
AGTCTAG18855.1141014E-639.7023472
CAGTCTA17351.4603575E-435.945691
GACCTTA13950.002173888535.765321
GCGTGTA14150.002331150235.25981
CGCATTG18752.304125E-433.2617452
GCGTGAT15000.003103034333.261741
CGCCTAA15150.003258065632.932422
GAGTTCA36151.4426405E-831.0534931
GAGCCTA28402.3893317E-630.743691
CGTTATA24450.030.6089672
CGGCCTA16650.005171679429.9655382
CAGCTAT33652.6693124E-729.6538561
ACGTATA33752.739598E-729.5659981
Produced by FastQC (version 0.11.5)