FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l05_n02_BG7180-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l05_n02_BG7180-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10666854
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG18459.4700226E-846.4211652
GCGCATA20101.8567516E-742.6104741
GCGTACT23953.009414E-530.6520921
GCGTACA20704.6087397E-429.5538481
ACGTGTA21155.227071E-428.9250431
CGTGTAA17050.00638167628.7044982
CGGGCAA30454.778978E-628.1271132
CGGTTTA26556.0778526E-527.650382
GCGGGCA40555.3147232E-827.1560131
ACGCATA18350.00913405226.6709381
TAGGACG19000.025.7585121
TCGGGCA29951.3789852E-424.5114381
CGCATAT26200.001823494323.3497982
GCGTCAT26250.001843831423.305321
GAGTCCA42502.4226665E-623.031141
GCCTACA27000.002171984222.6579511
AGACTCT38052.6890199E-522.509081
CGGGCAT27600.002467810222.1653862
CGTACTA27850.002600468221.9664152
GCGTATA17301.4364487E-621.2172721
Produced by FastQC (version 0.11.5)