FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l05_n02_BG715-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l05_n02_BG715-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6440305
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATA13004.088015E-757.2781561
GCGTGTT10004.3284037E-449.641071
CGCTATA12200.001152740340.6894042
GAGTCTA17401.5285597E-435.661691
ACGTGCA14700.002879087833.7694321
GCGCTAT11101.24752005E-533.541261
AAGTCTA29951.00208126E-733.1492961
CGTATAT19002.5628763E-432.65862
CAGGGTG15500.003732526432.0264971
GCGTTCA15850.0041636531.3192881
GCGTATG15850.0041636531.3192881
CGCTCTA19903.3622942E-431.181581
GAGTGTT16000.004360056531.025671
GACTGTA12004.0009796E-531.025671
AGTCTAG16800.00553497129.5482582
CGTATAA18000.00775259927.5783712
GAGCCTT24200.001055768225.6410481
AGCCTTA24400.001107689925.4308762
GCGATCT25750.00151604824.0976091
GACCCTT21102.5465852E-1023.5265731
Produced by FastQC (version 0.11.5)