FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l05_n02_BG715-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l05_n02_BG715-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15391841
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTAG34957.2759576E-1238.7425272
CGGATAG30953.614332E-935.7951622
GCGTTCT44703.6379788E-1233.0457951
GCGTATA26350.032.7009161
CGTACTA39551.1459633E-931.124042
GCGATCA36101.6219929E-830.688651
AAGTGTC29057.876224E-1029.6615871
GAGTCTA37702.4734618E-829.3862131
CGCTGTA21203.7823338E-729.0319792
CGTGTAT38402.9576768E-828.8505272
GCGTACA25654.6142115E-528.7942871
CGCTATA21650.028.4285412
GCGTTCA40404.8423317E-827.4222851
CGACTTA22557.343536E-427.2939191
CGGGCTA27450.026.9061372
CGTTCTA50755.6024874E-1026.6808172
CCGTGTA23509.344829E-426.1905481
CGTCCTA23509.344829E-426.1905482
GAGCCTT52150.025.9645541
GCGTGTA28709.914671E-525.7342681
Produced by FastQC (version 0.11.5)