FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l05_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l05_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16312439
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACACGT5400.025.9918226-27
TCAACGA9850.025.5759522-23
CGTTTCG5150.025.15737222-23
TTACGTT6900.024.51449224-25
TAGTCGT6950.023.81984126-27
TAACGGG3200.023.61801724-25
TCGACAG6050.023.19975324-25
GTCGACA7350.023.01360524-25
TACAGCG7000.022.62145226-27
CGTTACG6000.022.1932322-23
GCGAGTA6800.021.69946924-25
CGAGTTT7800.021.68548226-27
GTCGCGA9800.021.66685722-23
ACGTTAG6350.021.53673622-23
ACGATTG7550.021.45020326-27
TTAGCGC7000.021.07948324-25
TAGGTCA7350.021.054626-27
TAACGTC6500.021.03993624-25
AGTGCGC4500.020.79385424-25
TACGTCA7100.020.78219226-27
Produced by FastQC (version 0.11.5)