FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l05_n01_DHA60-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l05_n01_DHA60-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23077753
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG33600.017.732215
CGTCTTC566150.015.09261194-95
CCGTCTT633650.015.06295694-95
GCCGTCT767350.015.0507394-95
GTCTTCT482600.014.84392394-95
TATACCG80650.014.6604755
TGCCGTC927750.014.34537394-95
GTCTAGG67450.013.4231
TTCTGCT298000.013.414515594-95
CTTCTGC356150.012.71565994-95
CCTAGAC69400.012.5795643
TAGAGCG83950.012.5437275
TCTTCTG422000.012.515491594-95
ATGCCGT1022000.012.43491594-95
TATACTG98400.012.0159275
CTAGACA105200.011.8550344
GTATAAG93300.011.6843841
ATAGACG40350.011.6764654
GTCTATG88900.011.6391631
TATGCCG1165400.011.46160992-93
Produced by FastQC (version 0.11.5)