FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l05_n01_DHA180-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l05_n01_DHA180-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11030287
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG15700.019.7111875
TATACCG41200.016.480025
CCTAGAC35650.015.2899963
GTCTAGG31700.015.0099271
GTCTATG42300.014.306441
ACTGTCC28850.014.0890718
GTCTTCT219850.013.74069294-95
CGTCTTC249650.013.64544894-95
GCCGTCT318300.013.35083794-95
CTAGACA52350.013.2359024
CCGTCTT271400.013.21566294-95
TGCCGTC381300.013.21040694-95
GACTGCG15050.013.1970677
TTCTGCT138950.013.11431994-95
GTCCTGG31250.012.4173771
TAGAGCG42300.012.338885
CTTCTGC169750.012.31306494-95
TCTTCTG192150.012.29596394-95
ATGCCGT419200.012.208878594-95
TAGACAC45150.012.1738195
Produced by FastQC (version 0.11.5)