FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l05_n01_DHA15-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l05_n01_DHA15-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10214226
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG14950.015.4402175
GTCTAGG32650.014.4249341
ACTGTCC26050.013.6463378
TATACTG43450.013.387685
GTCTTCT181700.012.70431894-95
GACAGTG29500.012.6764047
GTGTATG50950.012.5063841
TATACCG38650.012.42248255
GTGTTAG22350.012.3956861
CCTAGAC31400.012.3517783
GCCGTCT241350.012.33811194-95
CGTCTTC198950.012.33376394-95
TGCCGTC289050.012.33049794-95
TAGAGCG42500.012.0575375
GTACCGT28150.011.9717716
CCGTCTT211850.011.9423194-95
GTCTATG37650.011.8960721
CTAGACA45450.011.8857194
CCAGGGT23950.011.7599153
TTCTGCT116250.011.59647894-95
Produced by FastQC (version 0.11.5)