FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l05_n01_BG760-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l05_n01_BG760-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8105255
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG13050.018.758885
GTCTTCT154350.014.99314594-95
TAGAGCG33200.014.8863165
CGTCTTC176900.014.5833494-95
GTACCGT20100.014.2470136
TATACCG29050.014.1509455
CTAGACA39550.014.1329894
GTCTAGG26500.014.1208571
GTCTATG31350.013.9993551
CCGTCTT191900.013.97297694-95
GCCGTCT228850.013.82615294-95
TAAGGTG41300.013.7561415
TGCCGTC280000.013.63472894-95
CCTAGAC27850.013.601493
AGTACCG25500.013.5850935
GACGGTC15600.013.3240077
TATACTG32950.013.0367425
TCTTCTG131750.012.98970494-95
ATAGACG14800.012.7972684
TCTAGAC28900.012.7876273
Produced by FastQC (version 0.11.5)