FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l05_n01_BG760-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l05_n01_BG760-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17990477
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG26050.019.4987355
GTCTAGG58200.016.9818381
CCTAGAC60550.014.9490673
TATACCG62350.014.7379855
GCCGTCT550600.014.47200394-95
GTACCGT40300.014.0933146
ACTGTCC41650.013.9698438
GTCTTCT365150.013.822885594-95
TAGAGCG61100.013.7547185
TGCCGTC658750.013.75046994-95
CCGTCTT462300.013.71002794-95
CGTCTTC414350.013.59697694-95
AGTACCG54250.013.5337725
TTCTGCT238800.013.42069594-95
GTCTATG70950.013.3442871
CTTCTGC275400.013.23009494-95
CTAGACA77350.012.8962074
TCTTCTG327150.012.64768294-95
TACCGTG47950.012.6159127
TATACTG84100.012.3540125
Produced by FastQC (version 0.11.5)