FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l05_n01_BG75D-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l05_n01_BG75D-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14481499
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG45450.015.9529761
TATACCG52700.014.5444385
CCTAGAC47700.014.5219973
TAGACGG19850.013.9569415
GCCGTCT278150.013.7790194-95
TGCCGTC338400.013.67958894-95
GTCTTCT216550.013.46586294-95
CGTCTTC226650.012.96766294-95
CTAGACA73800.012.8901164
CCGTCTT242200.012.2876294-95
TCTTCTG192000.012.22950194-95
ATGCCGT368300.012.01736994-95
ACTGTCC38600.011.7230678
TAAGGTG76650.011.3854225
GACAGTG38900.011.2765177
CTAGACT35500.011.1870634
TAGAGGT61100.011.1857854
TAGACCG32850.011.10430055
CTGTTCG63250.011.0965269
TATGCCG421850.011.02266692-93
Produced by FastQC (version 0.11.5)