FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l05_n01_BG75D-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l05_n01_BG75D-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8792404
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGAC28950.014.2029573
GCCGTCT164900.013.99369494-95
TATACCG30100.013.9658625
GTACCGT21100.013.5734356
GTCTAGG29050.013.5181581
TGCCGTC201600.013.40549794-95
CCGTCTT142100.012.72785694-95
CGTCTTC129950.012.495872594-95
GACTGCG9400.012.2859147
TAGACGG12100.012.2168015
GACTGTG13650.012.1833077
GTCTATG33700.012.0641731
AGTACCG28450.012.0155125
ATGCCGT217400.011.71935294-95
GTCTTCT124700.011.66975394-95
GTCTAGT24150.011.669641
GTCTAGA31750.011.6410191
CTAGACA41450.011.4801494
GTATAAG39050.011.2395091
GTGTATG44700.011.0591311
Produced by FastQC (version 0.11.5)