FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l05_n01_BG7180-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l05_n01_BG7180-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9779360
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG13600.017.6648675
GTCTATG39400.016.5355991
TATACCG37000.016.1077145
GTCTAGG32950.015.5655541
GTACCGT25350.015.1265346
GACAGTG25350.014.7623417
GACGGTC15050.014.7350947
ACTGTCC25250.014.2718878
AGTACCG31700.013.9912955
GCCGTCT189300.013.64322894-95
CCTAGAC31900.013.1807783
TAGAGCG43950.013.1400535
CCGTCTT167200.013.0977994-95
CGTCTTC149950.013.00235394-95
TGCCGTC226900.012.84845394-95
GTCTTCT142600.012.84634694-95
GTGTATG49700.012.4579171
GGACTGC25300.012.4173156
TACCGTG29100.012.3836827
CCAGGGT21850.012.2649813
Produced by FastQC (version 0.11.5)