FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l05_n01_BG7180-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l05_n01_BG7180-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10666854
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC321150.013.54378994-95
GCCGTCT262150.013.45676694-95
TATACCG34100.013.2695335
TATACTG50500.012.3431615
CCGTCTT220100.012.29347994-95
CGTCTTC205500.011.98725894-95
GTCTTCT185250.011.96410594-95
GTGTATG49200.011.7330341
ATGCCGT343750.011.43771294-95
GTCTAGG32750.011.4219211
TATGCCG376850.011.40710292-93
ACTGTCC28800.010.5812638
GTCTATG39300.010.3408341
TAGACGG16600.010.2914755
AGACTGC24400.010.0289576
GTACCGT26850.09.9736346
GACTGCG19950.09.9520287
CTATACT55050.09.8981844
TCTTCTG168300.09.87678594-95
ACTGAGC53850.09.8604828
Produced by FastQC (version 0.11.5)