FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l05_n01_BG715-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l05_n01_BG715-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6440305
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG20800.014.88273051
TATACCG22750.014.213585
GCCGTCT151600.014.19962594-95
TGCCGTC180950.013.31330694-95
CCGTCTT128250.013.18298994-95
GTCTTCT108100.013.01223294-95
TAGACGG10050.012.8700075
GTGTTAC11850.012.8666871
GTGTATG32650.012.7359231
CCTAGAC20550.012.5902353
GGACTGC21000.012.5380186
CGTCTTC118400.012.48499994-95
GTCTATG26350.012.0987241
TAAGGTG30750.012.0179855
GTATAGG27800.011.966271
ATGCCGT196100.011.87252794-95
TAGAGCG25650.011.7061175
GTGTAGG17200.011.2821531
ACTGAGC31700.011.220578
TCTTCTG95850.011.20523694-95
Produced by FastQC (version 0.11.5)