FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l05_n01_BG715-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l05_n01_BG715-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15391841
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG48300.017.4984131
CCTAGAC45400.016.2755743
GTCTATG59500.015.7570111
TAGAGCG56450.014.8060865
TGCTTGA174350.014.34275194-95
TATACCG49900.014.2510145
TCTGCTT283600.014.23184494-95
TAGACGG24400.014.19374755
TTCTGCT323600.014.14233594-95
GCCGTCT742600.014.06674594-95
ACTGTCC40500.014.0247148
CTGCTTG216600.013.79436894-95
CTAGACA67050.013.7752854
GCTTGAA137800.013.77363594-95
GTATAGA65450.013.760041
TATACTG71450.013.5073355
TGCCGTC882450.013.3027594-95
CCGTCTT619400.013.20766294-95
GACAGTG48850.012.6672637
GTGTATG78850.012.5344951
Produced by FastQC (version 0.11.5)