FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l04_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l04_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14999216
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT5350.015.6214876
TATACTG7800.014.8814337
GTCTATG5603.929017E-1014.7526073
TAACGTG3203.6416513E-614.5093967
GTGTTAC3603.5526413E-513.7690993
CACTGAG8750.013.2651599
GTCTAGT5205.195816E-712.7099383
GCCTAGT3050.002200025812.6404863
CCGTATC26550.012.58670694-95
GTGTAAG3150.002802530312.2392013
TAGAACC9700.011.9666326
GTCCTAG6002.2591303E-711.9332193
GTTCTAA6959.891664E-911.8869923
GGACTAG4251.6791341E-411.6632383
GTCTTAG4352.0843238E-411.3951173
CCTAGGC4952.2229378E-611.268275
GTCCTAA5401.094391E-511.2192673
ATACTGG6252.5707777E-811.1430678
AGTACCG4651.3851695E-510.9834577
CGTATCA21200.010.83799692-93
Produced by FastQC (version 0.11.5)