FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l04_n02_DHA5D-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l04_n02_DHA5D-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16199968
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC197950.016.04940294-95
GCCGTAT287500.014.42439594-95
CGCCGTA339650.013.96561894-95
CGTATCA163300.012.84674694-95
TGGTCGC609150.012.1613192-93
GGTCGCC632700.011.81785494-95
GTCGCCG691400.011.33812394-95
TCGCCGT470600.011.19398894-95
GTGGTCG669150.010.69627892-93
TCTCGGG100200.010.49159294-95
TATACCG55450.010.1313597
GTGTTAG34900.010.1234743
GGTGGTC739650.010.10957790-91
GTATCAT171900.010.04402994-95
TAGAGCG52650.09.6119617
TATCATT182100.09.58338294-95
TCGGTGG834150.09.55984488-89
CGGTGGT756900.09.53576790-91
CTAGACA70450.09.4241376
TAAGACT55700.09.3357546
Produced by FastQC (version 0.11.5)