FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l04_n02_DHA5D-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l04_n02_DHA5D-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11293309
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG14550.017.2315887
CCGTATC113950.015.29442694-95
GTCTAGG33150.013.9811133
GACTGCG14800.013.8024639
TATACCG43750.012.9472137
CCTAGAC32000.012.6366555
CGTATCA105650.012.63007594-95
GTCCTGG28000.012.2174243
CCAGGGT26250.012.0404435
GTGTATG56750.011.37538053
TGGTCGC298500.011.36680892-93
GTCTATG42950.011.3048673
GGTCGCC310950.011.1423594-95
GTCGCCG325000.010.97483794-95
TAGACCG24650.010.9246027
GGACTGC31050.010.9155158
TAGAGCG41700.010.800167
CTAGACA52650.010.7587966
CGCCGTA196100.010.69789794-95
GACTGTG17450.010.642179
Produced by FastQC (version 0.11.5)