FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l04_n02_CUI60-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l04_n02_CUI60-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12450633
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG18500.017.5679937
GTCTAGG39750.017.5263213
TATACCG43600.015.0151087
TAGAGCG48950.014.7967997
TAAGGCG22550.014.4127667
GTCTATG50200.014.3184833
CCGTATC193450.014.11940394-95
CCTAGAC42650.013.9497315
GTACCGT29600.013.80297858
CTAGACA57150.013.6487896
CTAAGGT53950.012.5656
GACAGTG40300.012.5570499
CGTATCA168800.012.51058494-95
CACTGAG74100.012.3428139
GCCTTAA29600.012.3284823
AGACACT37900.012.2501858
TAAGGTG63300.012.2492267
TAGAGGT49950.012.1769056
GTATAAG51900.012.1449263
ATAGACG22250.012.1031686
Produced by FastQC (version 0.11.5)