FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l04_n02_CUI60-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l04_n02_CUI60-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15001103
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG19650.017.7208887
CCGTATC188000.016.55311894-95
CGTATCA170300.014.47167394-95
GTCTAGG47700.013.3047233
TATACCG57700.012.5526537
GTATCAT169100.012.22764994-95
GTGTTAC21850.012.123173
GTGTTAT39400.011.6254093
GCCGTAT280750.011.5970394-95
CCTAGAC48550.011.4879665
GTATAAG67300.011.4799343
GACGGTC24350.011.4395799
GTCGCCG493250.011.39504194-95
CTAGACA70500.011.1956546
TATACTG69100.011.1536457
TGGTCGC483900.011.13646592-93
CGCCGTA309300.011.1117994-95
GGTCGCC486900.011.05750394-95
TCGCCGT368450.011.05369494-95
GACAGGC41400.010.989649
Produced by FastQC (version 0.11.5)