FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l04_n02_CUI5D-W-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l04_n02_CUI5D-W-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19045572
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG27150.017.613787
CACTGAG89550.014.9308529
TAGAGCG60400.014.6818767
GTCTAGG58000.014.4816453
CCGTATC497800.014.34894194-95
ATCATTA321800.014.23531894-95
TATCATT375500.014.17099194-95
GTATCAT382050.014.15885694-95
GTCTATG67650.013.9678833
TATACCG63700.013.9212767
CGTATCA416200.013.73867194-95
GTGTATG88050.013.3048133
GTATAAG75800.013.267973
GTACCGT40550.012.8234668
CCTAGAC61400.012.7930565
GCCGTAT640600.012.51242494-95
AGTACCG48300.012.4001717
GTATAGA83900.012.382213
GTCTTAG50450.012.3771183
TCATTAA266450.012.28031594-95
Produced by FastQC (version 0.11.5)