FastQCFastQC Report
Tue 17 Dec 2019
CCY3RACXX_l04_n02_CUI5D-S-R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCCY3RACXX_l04_n02_CUI5D-S-R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13900151
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG18850.016.7487757
CCGTATC152250.015.0898794-95
CGTATCA135400.012.837213594-95
GACGGTC23400.011.705729
TATACCG50350.011.5264617
GCCGTAT236900.011.48074694-95
CGCCGTA258100.011.3829194-95
GTCTAGG42450.011.1972123
CTAGACA65300.011.16288956
GGTCGCC401100.011.05644894-95
GTATCAT143250.011.01594894-95
TGGTCGC401300.010.98205892-93
GTGTTAG25550.010.816043
GTCGCCG426250.010.81240894-95
TCGCCGT315500.010.63595694-95
CCTAGAC45550.010.6127225
GTGCTAG46400.010.12488653
GTCCTGG33150.010.0036133
GTCTATG52850.09.93499853
TAGAGCG48500.09.8600927
Produced by FastQC (version 0.11.5)